Detection of Bovine Viral Diarrhoea Virus p80 Protein in Subpopulations of Bovine Leukocytes

Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes.

Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood m...

Detection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

The occurrence of bovine virus diarrhoea/muscosal disease in Iran

Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)

Bovine Viral Diarrhea virus (BVDV) is an important viral pathogen of cattle causing several clinical syndromes. There are usually no pathognomonic clinical signs of BVDV infection. Diagnostic investigations therefore rely on serological detection and virus isolation. Nonstructural protein 3 (NS3) as immunogenic protein of BVDV is genetically and antigenically conserved among different isolates....

Prevalence of bovine viral diarrhoea virus antibodies in India.

A study was undertaken regarding the prevalence of bovine viral diarrhoea virus (BVDV) antibodies in bovine sera which tested negative for rinderpest and peste des petits ruminants virus antibodies. The samples were collected between January 1996 and December 1997. A total of 439 samples (327 cattle and 112 buffalo from 17 states of India) were tested using a commercial enzyme-linked immunosorb...